VKORC1 ELISA Kit

Produktnummer ABIN5659967

Produktdetails VKORC1 ELISA Kit

Target: VKORC1 (Vitamin K Epoxide Reductase Complex, Subunit 1 (VKORC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). No significant cross-reactivity or interference between Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) and analogues was observed.

Sensitivität: 0.57 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu VKORC1

Target: VKORC1 (Vitamin K Epoxide Reductase Complex, Subunit 1 (VKORC1))

Andere Bezeichnung: Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1 Produkte)

Synonyme: MGC89620 ELISA Kit, BP1071 ELISA Kit, CG33544 ELISA Kit, Dmel\\CG33544 ELISA Kit, HDC06808 ELISA Kit, VKORC1 ELISA Kit, EDTP308 ELISA Kit, IMAGE3455200 ELISA Kit, MST134 ELISA Kit, MST576 ELISA Kit, VKCFD2 ELISA Kit, VKOR ELISA Kit, D7Wsu86e ELISA Kit, vitamin K epoxide reductase complex, subunit 1 ELISA Kit, vitamin K epoxide reductase complex subunit 1 ELISA Kit, Vitamin-K epoxide reductase ELISA Kit, VKORC1 ELISA Kit, vkorc1 ELISA Kit, Vkor ELISA Kit, Vkorc1 ELISA Kit

Hintergrund:

Gene Name: Vitamin K Epoxide Reductase Complex Subunit 1

Gene Aliases: VKCFD2, VKOR, Vitamin K Dependent Clotting Factors Deficiency 2, Vitamin K1 2,3-epoxide reductase subunit 1