Vitamin D2 CLIA Kit

Produktnummer ABIN5659962

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Diverse Spezies Vitamin D2.

Kurzübersicht für Vitamin D2 CLIA Kit (ABIN5659962)

Target: Vitamin D2

Reaktivität: Diverse Spezies

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 1.17 ng/mL - 300 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Vitamin D2 CLIA Kit

Untere Nachweisgrenze: 1.17 ng/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Vitamin D2 (VD2). No significant cross-reactivity or interference between Vitamin D2 (VD2) and analogues was observed.

Sensitivität: 0.45 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Vitamin D2 (VD2). A competitive inhibition reaction is launched between biotin labeled Vitamin D2 (VD2) and unlabeled Vitamin D2 (VD2) (Standards or samples) with the pre-coated antibody specific to Vitamin D2 (VD2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin D2 (VD2) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Vitamin D2 (VD2) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D2 (VD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D2 (VD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Vitamin D2

Hintergrund:

Gene Name: Vitamin D2

Gene Aliases: Ergocalciferol, Deltalin, Drisdol, Calcidol