Versican ELISA Kit

Produktnummer ABIN5659919

Produktdetails Versican ELISA Kit

Target: Versican (Vcan)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Versican (VS). No significant cross-reactivity or interference between Versican (VS) and analogues was observed.

Sensitivität: 32 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Versican (VS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Versican (VS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Versican (VS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Versican (VS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Versican

Target: Versican (Vcan)

Andere Bezeichnung: Versican (Vcan Produkte)

Synonyme: br146 ELISA Kit, cspg2 ELISA Kit, cspg2a ELISA Kit, sb:cb63 ELISA Kit, versican ELISA Kit, CSPG2 ELISA Kit, Cspg2 ELISA Kit, wgn ELISA Kit, ervr ELISA Kit, pg-m ELISA Kit, wgn1 ELISA Kit, Xversican ELISA Kit, dcan ELISA Kit, cspg2b ELISA Kit, fb99b07 ELISA Kit, wu:fb99b07 ELISA Kit, ERVR ELISA Kit, GHAP ELISA Kit, PG-M ELISA Kit, WGN ELISA Kit, WGN1 ELISA Kit, 5430420N07Rik ELISA Kit, 9430051N09 ELISA Kit, DPEAAE ELISA Kit, NG2 ELISA Kit, PG-M(V0) ELISA Kit, PG-M(V1) ELISA Kit, hdf ELISA Kit, versican a ELISA Kit, versican ELISA Kit, versican b ELISA Kit, vcana ELISA Kit, VCAN ELISA Kit, Vcan ELISA Kit, vcan ELISA Kit, vcanb ELISA Kit

Hintergrund:

Gene Name: Versican

Gene Aliases: CSPG2, VCAN, ERVR, PG-M, WGN1, Chondroitin Sulfate Proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding, Large fibroblast proteoglycan

Gen-ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process