VTCN1 ELISA Kit
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- Target Alle VTCN1 ELISA Kits anzeigen
- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1). No significant cross-reactivity or interference between V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) and analogues was observed.
- Sensitivität
- 0.058 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle VTCN1 ELISA Kits anzeigen
- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
- Andere Bezeichnung
- V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1 Produkte)
- Synonyme
- B7h4 ELISA Kit, B7s1 ELISA Kit, B7x ELISA Kit, BC032925 ELISA Kit, B7-H4 ELISA Kit, B7H4 ELISA Kit, B7S1 ELISA Kit, B7X ELISA Kit, B7h.5 ELISA Kit, PRO1291 ELISA Kit, VCTN1 ELISA Kit, RGD1311204 ELISA Kit, V set domain containing T cell activation inhibitor 1 L homeolog ELISA Kit, V-set domain containing T cell activation inhibitor 1 ELISA Kit, V-set domain containing T-cell activation inhibitor 1 ELISA Kit, V set domain containing T cell activation inhibitor 1 ELISA Kit, vtcn1.L ELISA Kit, VTCN1 ELISA Kit, vtcn1 ELISA Kit, Vtcn1 ELISA Kit
- Hintergrund
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Gene Name: V-Set Domain Containing T-Cell Activation Inhibitor 1
Gene Aliases: B7-H4, B7H4, B7S1, B7X, B7h.5, VCTN1, T-cell costimulatory molecule B7x, Immune costimulatory protein B7-H4
- Gen-ID
- 79679
- UniProt
- Q7Z7D3
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