Uridine Triphosphate ELISA Kit
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- Target
- Uridine Triphosphate
- Reaktivität
- Diverse Spezies
- Nachweismethode
- Colorimetric
- Methodentyp
- Competition ELISA
- Detektionsbereich
- 61.73 ng/mL - 5000 ng/mL
- Untere Nachweisgrenze
- 61.73 ng/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Uridine Triphosphate (UTP). No significant cross-reactivity or interference between Uridine Triphosphate (UTP) and analogues was observed.
- Sensitivität
- 26.5 ng/mL
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Uridine Triphosphate ELISA Kit
Reaktivität: Diverse Spezies Colorimetric Competition ELISA 61.7 ng/mL - 5000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 h
- Plattentyp
- Pre-coated
- Protokoll
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Uridine Triphosphate (UTP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Uridine Triphosphate (UTP) and unlabeled Uridine Triphosphate (UTP) (Standards or samples) with the pre-coated antibody specific to Uridine Triphosphate (UTP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Uridine Triphosphate (UTP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Uridine Triphosphate (UTP) in the sample.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uridine Triphosphate (UTP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uridine Triphosphate (UTP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target
- Uridine Triphosphate
- Hintergrund
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Gene Name: Uridine Triphosphate
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