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Uracil Phosphoribosyltransferase (UPP) ELISA Kit

UPP Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5659763
  • Target Alle Uracil Phosphoribosyltransferase (UPP) Produkte
    Uracil Phosphoribosyltransferase (UPP)
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Uracil Phosphoribosyltransferase (UPRT). No significant cross-reactivity or interference between Uracil Phosphoribosyltransferase (UPRT) and analogues was observed.
    Sensitivität
    0.057 ng/mL
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uracil Phosphoribosyltransferase (UPRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uracil Phosphoribosyltransferase (UPRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uracil Phosphoribosyltransferase (UPRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uracil Phosphoribosyltransferase (UPRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uracil Phosphoribosyltransferase (UPRT) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uracil Phosphoribosyltransferase (UPRT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Uracil Phosphoribosyltransferase (UPP) Produkte
    Uracil Phosphoribosyltransferase (UPP)
    Andere Bezeichnung
    Uracil Phosphoribosyltransferase (UPP Produkte)
    Synonyme
    FUR1 ELISA Kit, UPP ELISA Kit, PYRIMIDINE R ELISA Kit, PYRR ELISA Kit, uracil phosphoribosyltransferase ELISA Kit, uracil phosphoribosyltransferase homolog ELISA Kit, uracil phosphoribosyltransferase ELISA Kit, UPRT ELISA Kit, UPP ELISA Kit
    Hintergrund

    Gene Name: Uracil Phosphoribosyltransferase

    Gene Aliases: FUR1

    Gen-ID
    139596
    UniProt
    Q96BW1
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