TSG101 ELISA Kit

Produktnummer ABIN5659699

Produktdetails TSG101 ELISA Kit

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tumor Susceptibility Gene 101 (TSG101). No significant cross-reactivity or interference between Tumor Susceptibility Gene 101 (TSG101) and analogues was observed.

Sensitivität: 0.114 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Susceptibility Gene 101 (TSG101). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Susceptibility Gene 101 (TSG101). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Susceptibility Gene 101 (TSG101), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Susceptibility Gene 101 (TSG101) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Andere Bezeichnung: Tumor Susceptibility Gene 101 (TSG101 Produkte)

Synonyme: CG9712 ELISA Kit, DmTSG101 ELISA Kit, Dmel\\CG9712 ELISA Kit, ESCRT-I ELISA Kit, Tsg101 ELISA Kit, burs ELISA Kit, dTSg101 ELISA Kit, dTsg101 ELISA Kit, dVps23 ELISA Kit, ept ELISA Kit, tsg101 ELISA Kit, vps23 ELISA Kit, TSG101 ELISA Kit, MGC76193 ELISA Kit, TSG10 ELISA Kit, VPS23 ELISA Kit, AI255943 ELISA Kit, CC2 ELISA Kit, Rw ELISA Kit, zgc:86926 ELISA Kit, tumor susceptibility 101 ELISA Kit, Tumor susceptibility gene 101 ELISA Kit, Tumor Susceptibility Gene homolog ELISA Kit, tumor susceptibility gene 101 ELISA Kit, tumor susceptibility 101a ELISA Kit, tumor susceptibility 101 L homeolog ELISA Kit, TSG101 ELISA Kit, tsg-101 ELISA Kit, tsg101 ELISA Kit, Tsg101 ELISA Kit, tsg101a ELISA Kit, tsg101.L ELISA Kit

Hintergrund:

Gene Name: Tumor Susceptibility Gene 101

Gene Aliases: TSG10, VPS23, Tumor Susceptibility Gene 10, ESCRT-I complex subunit TSG101