TPM3 ELISA Kit

Produktnummer ABIN5659551

Produktdetails TPM3 ELISA Kit

Target: TPM3 (Tropomyosin 3 (TPM3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tropomyosin 3 (TPM3). No significant cross-reactivity or interference between Tropomyosin 3 (TPM3) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomyosin 3 (TPM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tropomyosin 3 (TPM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomyosin 3 (TPM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tropomyosin 3 (TPM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tropomyosin 3 (TPM3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tropomyosin 3 (TPM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TPM3

Target: TPM3 (Tropomyosin 3 (TPM3))

Andere Bezeichnung: Tropomyosin 3 (TPM3 Produkte)

Synonyme: CAPM1 ELISA Kit, CFTD ELISA Kit, NEM1 ELISA Kit, OK/SW-cl.5 ELISA Kit, TM-5 ELISA Kit, TM3 ELISA Kit, TM30 ELISA Kit, TM30nm ELISA Kit, TM5 ELISA Kit, TPMsk3 ELISA Kit, TRK ELISA Kit, hscp30 ELISA Kit, cb674 ELISA Kit, fa17b11 ELISA Kit, fb83b01 ELISA Kit, fk41e01 ELISA Kit, hm:zeh0298 ELISA Kit, wu:fa17b11 ELISA Kit, wu:fb83b01 ELISA Kit, wu:fk41e01 ELISA Kit, zeh0298 ELISA Kit, zgc:77592 ELISA Kit, TPM3 ELISA Kit, Tm5NM ELISA Kit, Tpm-5 ELISA Kit, Tpm5 ELISA Kit, Trop-5 ELISA Kit, gamma-TM ELISA Kit, hTM30nm ELISA Kit, hTMnm ELISA Kit, tm3 ELISA Kit, tpm3 ELISA Kit, tpm3.L ELISA Kit, tropomyosin 3 ELISA Kit, tropomyosin 3, gamma ELISA Kit, tropomyosin 3 S homeolog ELISA Kit, TPM3 ELISA Kit, tpm3 ELISA Kit, Tpm3 ELISA Kit, tpm3.S ELISA Kit

Hintergrund:

Gene Name: Tropomyosin 3

Gene Aliases: NEM1, TRK, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TPMsk3, Hscp30, Gamma-tropomyosin

Gen-ID: 59069

UniProt: P21107