TFF1 ELISA Kit

Produktnummer ABIN5659518

Produktdetails TFF1 ELISA Kit

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1). No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) and analogues was observed.

Sensitivität: 27 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 1 (TFF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trefoil Factor 1 (TFF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trefoil Factor 1 (TFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trefoil Factor 1 (TFF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Andere Bezeichnung: Trefoil Factor 1 (TFF1 Produkte)

Synonyme: BCEI ELISA Kit, D21S21 ELISA Kit, HP1.A ELISA Kit, HPS2 ELISA Kit, pNR-2 ELISA Kit, pS2 ELISA Kit, Bcei ELISA Kit, PS2 ELISA Kit, bcei ELISA Kit, d21s21 ELISA Kit, hp1.a ELISA Kit, hps2 ELISA Kit, p1 ELISA Kit, p1-a ELISA Kit, pnr-2 ELISA Kit, ps2 ELISA Kit, tff1 ELISA Kit, xP1 ELISA Kit, xp1-L ELISA Kit, xp1 ELISA Kit, TFF1 ELISA Kit, Ps2 ELISA Kit, trefoil factor 1 ELISA Kit, trefoil factor 1 S homeolog ELISA Kit, trefoil factor 1, gene 2 S homeolog ELISA Kit, TFF1 ELISA Kit, Tff1 ELISA Kit, tff1.S ELISA Kit, tff1.2.S ELISA Kit, LOC100358238 ELISA Kit

Hintergrund:

Gene Name: Trefoil Factor 1

Gene Aliases: BCEI, HP1.A, HPS2, PS2, PNR2, Breast Cancer,Estrogen-Inducible Sequence Expressed In, Breast cancer estrogen-inducible protein, Polypeptide P1.A

Gen-ID: 403491

UniProt: Q863T4

Pathways: EGFR Signaling Pathway