TMSB4X ELISA Kit

Produktnummer ABIN5659216

Produktdetails TMSB4X ELISA Kit

Target: TMSB4X (Thymosin beta-4 (TMSB4X))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 2.47 ng/mL - 200 ng/mL

Untere Nachweisgrenze: 2.47 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Thymosin Beta 4 (Tb4). No significant cross-reactivity or interference between Thymosin Beta 4 (Tb4) and analogues was observed.

Sensitivität: 0.91 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymosin Beta 4 (Tb4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymosin Beta 4 (Tb4) and unlabeled Thymosin Beta 4 (Tb4) (Standards or samples) with the pre-coated antibody specific to Thymosin Beta 4 (Tb4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymosin Beta 4 (Tb4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymosin Beta 4 (Tb4) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymosin Beta 4 (Tb4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymosin Beta 4 (Tb4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TMSB4X

Target: TMSB4X (Thymosin beta-4 (TMSB4X))

Andere Bezeichnung: Thymosin Beta 4 (TMSB4X Produkte)

Synonyme: FX ELISA Kit, PTMB4 ELISA Kit, TB4X ELISA Kit, TMSB4 ELISA Kit, Ptmb4 ELISA Kit, Tb4 ELISA Kit, tmsb4 ELISA Kit, TMSB4X ELISA Kit, thymosin beta 4, X-linked ELISA Kit, thymosin, beta 4, X chromosome ELISA Kit, thymosin beta 4, X-linked L homeolog ELISA Kit, thymosin, beta 4-like ELISA Kit, thymosin beta 4 pseudogene ELISA Kit, TMSB4X ELISA Kit, Tmsb4x ELISA Kit, tmsb4x.L ELISA Kit, TMSB4 ELISA Kit, LOC100339394 ELISA Kit, LOC100860922 ELISA Kit

Hintergrund:

Gene Name: Thymosin Beta 4

Gene Aliases: TMSB4X, FX, PTMB4, TB4X, TMSB4, Thymosin,Beta 4,X-Linked, TB-500, Seraspenide, Hematopoietic system regulatory peptide

Gen-ID: 7114

UniProt: P62328

Pathways: Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Smooth Muscle Cell Migration