TK1 ELISA Kit

Produktnummer ABIN5659208

Produktdetails TK1 ELISA Kit

Target: TK1 (Thymidine Kinase 1, Soluble (TK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Thymidine Kinase 1, Soluble (TK1). No significant cross-reactivity or interference between Thymidine Kinase 1, Soluble (TK1) and analogues was observed.

Sensitivität: 6.3 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Kinase 1, Soluble (TK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Kinase 1, Soluble (TK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Kinase 1, Soluble (TK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Kinase 1, Soluble (TK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TK1

Target: TK1 (Thymidine Kinase 1, Soluble (TK1))

Andere Bezeichnung: Thymidine Kinase 1, Soluble (TK1 Produkte)

Synonyme: fi19b06 ELISA Kit, zgc:55362 ELISA Kit, wu:fi19b06 ELISA Kit, TK1 ELISA Kit, tk2 ELISA Kit, D530002A18Rik ELISA Kit, Tk-1 ELISA Kit, Tk1a ELISA Kit, Tk1b ELISA Kit, TK2 ELISA Kit, Tk ELISA Kit, TK ELISA Kit, thymidine kinase 1, soluble ELISA Kit, thymidine kinase 1 ELISA Kit, thymidine kinase, cytosolic ELISA Kit, Thymidine kinase, cytosolic ELISA Kit, temporal expression: early ELISA Kit, thymidine kinase ELISA Kit, Tk thymidine kinase ELISA Kit, tk1 ELISA Kit, TK1 ELISA Kit, EDI_049980 ELISA Kit, kith ELISA Kit, J2R ELISA Kit, HVT031 ELISA Kit, Tk1 ELISA Kit, tk ELISA Kit

Hintergrund:

Gene Name: Thymidine Kinase 1, Soluble

Gene Aliases: TK2, Thymidine kinase, cytosolic

Gen-ID: 7083

UniProt: P04183