Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CD90 ELISA Kit

THY1 Reaktivität: Maus Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Produktnummer ABIN5659199
  • Target Alle CD90 (THY1) ELISA Kits anzeigen
    CD90 (THY1)
    Reaktivität
    • 2
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    12.5 pg/mL - 800 pg/mL
    Untere Nachweisgrenze
    12.5 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Thy1 Cell Surface Antigen (Thy1). No significant cross-reactivity or interference between Thy1 Cell Surface Antigen (Thy1) and analogues was observed.
    Sensitivität
    5.7 pg/mL
    Featured
    Zu unserem meistverkauften THY1 ELISA Kit
    Top Product
    Discover our top product THY1 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thy1 Cell Surface Antigen (Thy1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thy1 Cell Surface Antigen (Thy1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thy1 Cell Surface Antigen (Thy1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thy1 Cell Surface Antigen (Thy1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thy1 Cell Surface Antigen (Thy1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thy1 Cell Surface Antigen (Thy1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CD90 (THY1) ELISA Kits anzeigen
    CD90 (THY1)
    Andere Bezeichnung
    Thy1 Cell Surface Antigen (THY1 Produkte)
    Synonyme
    CD90 ELISA Kit, CD7 ELISA Kit, T25 ELISA Kit, Thy-1 ELISA Kit, Thy-1.2 ELISA Kit, Thy1.1 ELISA Kit, Thy1.2 ELISA Kit, Thy-1 cell surface antigen ELISA Kit, thymus cell antigen 1, theta ELISA Kit, THY1 ELISA Kit, Thy1 ELISA Kit
    Hintergrund

    Gene Name: Thy1 Cell Surface Antigen

    Gene Aliases: CD90, CDw90, THY mocyte differentiation antigen 1

    Gen-ID
    21838
    UniProt
    P01831
    Pathways
    Cell-Cell Junction Organization
Sie sind hier:
Kundenservice