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Tetraspanin 1 (TSPAN1) ELISA Kit ELISA Kit

TSPAN1 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma
Produktnummer ABIN5659138
$971.15
Zzgl. Versandkosten $45.00
96 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 8 bis 12 Werktagen
  • Target
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Tetraspanin 1 (TSPAN1). No significant cross-reactivity or interference between Tetraspanin 1 (TSPAN1) and analogues was observed.
    Sensitivität
    0.074 ng/mL
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 1 (TSPAN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tetraspanin 1 (TSPAN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tetraspanin 1 (TSPAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetraspanin 1 (TSPAN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetraspanin 1 (TSPAN1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetraspanin 1 (TSPAN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target
    Andere Bezeichnung
    Tetraspanin 1 (TSPAN1 ELISA Kit Abstract)
    Hintergrund

    Gene Name: Tetraspanin 1

    Gene Aliases: NET-1, Tetraspanin TM4-C, Tetraspan NET-1

    Gen-ID
    10103
    UniProt
    O60635
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