CXCL12 ELISA Kit

Produktnummer ABIN5658970

Produktdetails CXCL12 ELISA Kit

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1). No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Sensitivität: 35 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stromal Cell Derived Factor 1 (SDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: Stromal Cell Derived Factor 1 (CXCL12 Produkte)

Synonyme: IRH ELISA Kit, PBSF ELISA Kit, SCYB12 ELISA Kit, SDF1 ELISA Kit, TLSF ELISA Kit, TPAR1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, Pbsf ELISA Kit, Scyb12 ELISA Kit, Sdf1 ELISA Kit, Tlsf ELISA Kit, Tpar1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, sdf-1 ELISA Kit, sdf1 ELISA Kit, xSDF-1 ELISA Kit, SDF-1 ELISA Kit, cxcl12 ELISA Kit, sdf-1a ELISA Kit, sdf1a ELISA Kit, wu:fa55e10 ELISA Kit, wu:fc16h12 ELISA Kit, wu:fj84c02 ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, C-X-C motif chemokine ligand 12 L homeolog ELISA Kit, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) ELISA Kit, CXCL12 ELISA Kit, cxcl12 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, cxcl12.L ELISA Kit, cxcl12a ELISA Kit

Hintergrund:

Gene Name: Stromal Cell Derived Factor 1

Gene Aliases: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12

Gen-ID: 24772

UniProt: Q9QZD1

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling