SHBG ELISA Kit

Produktnummer ABIN5658772

Produktdetails SHBG ELISA Kit

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Sex Hormone Binding Globulin (SHBG). No significant cross-reactivity or interference between Sex Hormone Binding Globulin (SHBG) and analogues was observed.

Sensitivität: 0.247 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Hormone Binding Globulin (SHBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Hormone Binding Globulin (SHBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Hormone Binding Globulin (SHBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Hormone Binding Globulin (SHBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SHBG

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Andere Bezeichnung: Sex Hormone Binding Globulin (SHBG Produkte)

Synonyme: ABP ELISA Kit, SBP ELISA Kit, TEBG ELISA Kit, Abpa ELISA Kit, shbgl ELISA Kit, wu:fb66h07 ELISA Kit, wu:fd55b08 ELISA Kit, zgc:123063 ELISA Kit, shbg ELISA Kit, sex hormone binding globulin ELISA Kit, sex hormone-binding globulin ELISA Kit, sex hormone binding globulin L homeolog ELISA Kit, SHBG ELISA Kit, Shbg ELISA Kit, shbg ELISA Kit, shbg.L ELISA Kit

Hintergrund:

Gene Name: Sex Hormone Binding Globulin

Gene Aliases: ABP, TEBG, SBP, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin

Gen-ID: 24775

UniProt: P08689

Pathways: Hormone Transport