SDPR ELISA Kit

Produktnummer ABIN5658757

Produktdetails SDPR ELISA Kit

Target: SDPR (Serum Deprivation Response (SDPR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Serum Deprivation Response Protein (SDPR). No significant cross-reactivity or interference between Serum Deprivation Response Protein (SDPR) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Deprivation Response Protein (SDPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Deprivation Response Protein (SDPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Deprivation Response Protein (SDPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Deprivation Response Protein (SDPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Deprivation Response Protein (SDPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Deprivation Response Protein (SDPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SDPR

Target: SDPR (Serum Deprivation Response (SDPR))

Andere Bezeichnung: Serum Deprivation Response Protein (SDPR Produkte)

Synonyme: CAVIN2 ELISA Kit, PS-p68 ELISA Kit, SDR ELISA Kit, cavin-2 ELISA Kit, sdr ELISA Kit, MGC84112 ELISA Kit, sdprb ELISA Kit, wu:fb39c08 ELISA Kit, wu:fk90b02 ELISA Kit, zgc:92231 ELISA Kit, caveolae associated protein 2 ELISA Kit, caveolae associated 2 ELISA Kit, caveolae associated protein 2 S homeolog ELISA Kit, serum deprivation response ELISA Kit, caveolae associated protein 2b ELISA Kit, CAVIN2 ELISA Kit, Cavin2 ELISA Kit, cavin2.S ELISA Kit, SDPR ELISA Kit, cavin2b ELISA Kit

Hintergrund:

Gene Name: Serum Deprivation Response Protein

Gene Aliases: PS-p68, SDR, Cavin-2, Phosphatidylserine Binding Protein