RUNX2 ELISA Kit

Produktnummer ABIN5658532

Produktdetails RUNX2 ELISA Kit

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Runt Related Transcription Factor 2 (RUNX2). No significant cross-reactivity or interference between Runt Related Transcription Factor 2 (RUNX2) and analogues was observed.

Sensitivität: 0.116 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt Related Transcription Factor 2 (RUNX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Runt Related Transcription Factor 2 (RUNX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Runt Related Transcription Factor 2 (RUNX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Runt Related Transcription Factor 2 (RUNX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Andere Bezeichnung: Runt Related Transcription Factor 2 (RUNX2 Produkte)

Synonyme: AML3 ELISA Kit, CBF-alpha-1 ELISA Kit, CBFA1 ELISA Kit, CCD ELISA Kit, CCD1 ELISA Kit, CLCD ELISA Kit, OSF-2 ELISA Kit, OSF2 ELISA Kit, PEA2aA ELISA Kit, PEBP2aA ELISA Kit, Cbf ELISA Kit, Cbfa-1 ELISA Kit, Cbfa1 ELISA Kit, LS3 ELISA Kit, Osf2 ELISA Kit, Pebp2a1 ELISA Kit, Pebpa2a ELISA Kit, runx2 ELISA Kit, RUNX2 ELISA Kit, ccd ELISA Kit, aml3 ELISA Kit, ccd1 ELISA Kit, osf2 ELISA Kit, cbfa1 ELISA Kit, pea2aa ELISA Kit, pebp2a1 ELISA Kit, pebp2a2 ELISA Kit, pebp2aa ELISA Kit, pebp2aa1 ELISA Kit, runt related transcription factor 2 ELISA Kit, runt-related transcription factor 2a ELISA Kit, runt-related transcription factor 2 ELISA Kit, runt related transcription factor 2 L homeolog ELISA Kit, RUNX2 ELISA Kit, runx2a ELISA Kit, Runx2 ELISA Kit, runx2 ELISA Kit, LOC703331 ELISA Kit, LOC100549663 ELISA Kit, runx2.L ELISA Kit

Hintergrund:

Gene Name: Runt Related Transcription Factor 2

Gene Aliases: AML3, CBFA1, CCD1, OSF2, PEBP2A, Acute myeloid leukemia 3 , Core-binding factor alpha-1, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 A

Gen-ID: 12393

UniProt: Q08775