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RBM38 ELISA Kit

RBM38 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Produktnummer ABIN5658527
  • Target Alle RBM38 ELISA Kits anzeigen
    RBM38 (RNA Binding Motif Protein 38 (RBM38))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of RNA Binding Motif Protein 38 (RBM38). No significant cross-reactivity or interference between RNA Binding Motif Protein 38 (RBM38) and analogues was observed.
    Sensitivität
    0.112 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 38 (RBM38). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RNA Binding Motif Protein 38 (RBM38). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 38 (RBM38), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNA Binding Motif Protein 38 (RBM38) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNA Binding Motif Protein 38 (RBM38) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNA Binding Motif Protein 38 (RBM38) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle RBM38 ELISA Kits anzeigen
    RBM38 (RNA Binding Motif Protein 38 (RBM38))
    Andere Bezeichnung
    RNA Binding Motif Protein 38 (RBM38 Produkte)
    Synonyme
    rnpc1 ELISA Kit, seb4b ELISA Kit, seb4d ELISA Kit, XSeb4R ELISA Kit, MGC89204 ELISA Kit, hsrnaseb ELISA Kit, YF-55 ELISA Kit, fi25e12 ELISA Kit, wu:fi25e12 ELISA Kit, zgc:92336 ELISA Kit, xseb4r ELISA Kit, HSRNASEB ELISA Kit, RNPC1 ELISA Kit, SEB4B ELISA Kit, SEB4D ELISA Kit, dJ800J21.2 ELISA Kit, Rnpc1 ELISA Kit, Seb4 ELISA Kit, Seb4l ELISA Kit, RNA binding motif protein 38 ELISA Kit, RNA binding motif protein 38 L homeolog ELISA Kit, rbm38 ELISA Kit, rbm38.L ELISA Kit, RBM38 ELISA Kit, Rbm38 ELISA Kit
    Hintergrund

    Gene Name: RNA Binding Motif Protein 38

    Gene Aliases: HSRNASEB, SEB4D, Seb4B, RNPC1, RNA-Binding Region(RNP1,RRM)Containing 1, CLL-associated antigen KW-5, ssDNA-binding protein SEB4

    Gen-ID
    55544
    UniProt
    Q9H0Z9
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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