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PKLR ELISA Kit

PKLR Reaktivität: Human Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5658320
  • Target Alle PKLR ELISA Kits anzeigen
    PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
    Reaktivität
    • 2
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    39.06 pg/mL - 2500 pg/mL
    Untere Nachweisgrenze
    39.06 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Pyruvate Kinase, Liver And RBC (PK). No significant cross-reactivity or interference between Pyruvate Kinase, Liver And RBC (PK) and analogues was observed.
    Sensitivität
    17 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Liver And RBC (PK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Liver And RBC (PK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Liver And RBC (PK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Liver And RBC (PK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Kinase, Liver And RBC (PK) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Kinase, Liver And RBC (PK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle PKLR ELISA Kits anzeigen
    PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
    Andere Bezeichnung
    Pyruvate Kinase, Liver And RBC (PKLR Produkte)
    Synonyme
    PK1 ELISA Kit, PKL ELISA Kit, PKR ELISA Kit, PKRL ELISA Kit, RPK ELISA Kit, Pklg ELISA Kit, wu:fd15e01 ELISA Kit, wu:fi37e08 ELISA Kit, pk1 ELISA Kit, PKLR ELISA Kit, Pk-1 ELISA Kit, Pk1 ELISA Kit, R-PK ELISA Kit, pklr ELISA Kit, pyruvate kinase L/R ELISA Kit, pyruvate kinase, liver and RBC ELISA Kit, pyruvate kinase, liver and RBC L homeolog ELISA Kit, pyruvate kinase liver and red blood cell ELISA Kit, pyruvate kinase PKLR-like ELISA Kit, PKLR ELISA Kit, Pklr ELISA Kit, pklr ELISA Kit, pklr.L ELISA Kit, LOC100621940 ELISA Kit
    Hintergrund

    Gene Name: Pyruvate Kinase, Liver And RBC

    Gene Aliases: PKLR, PyK, PK1, PKL, Pyruvate Kinase Isozymes R/L, Glutamic--pyruvic transaminase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

    Gen-ID
    5313
    UniProt
    P30613
    Pathways
    Ribonucleoside Biosynthetic Process
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