MPZ ELISA Kit

Produktnummer ABIN5658271

Dieses Ratte MPZ ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte MPZ zu quantifizieren.

Kurzübersicht für MPZ ELISA Kit (ABIN5658271)

Target: MPZ (Myelin Protein Zero (MPZ))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails MPZ ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Zero, Myelin (MPZ). No significant cross-reactivity or interference between Protein Zero, Myelin (MPZ) and analogues was observed.

Sensitivität: 0.122 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Protein Zero, Myelin (MPZ) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Protein Zero, Myelin (MPZ) and unlabeled Protein Zero, Myelin (MPZ) (Standards or samples) with the pre-coated antibody specific to Protein Zero, Myelin (MPZ). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Protein Zero, Myelin (MPZ) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Protein Zero, Myelin (MPZ) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MPZ

Target: MPZ (Myelin Protein Zero (MPZ))

Andere Bezeichnung: Protein Zero, Myelin

Hintergrund:

Gene Name: Protein Zero, Myelin

Gene Aliases: P0, DSS, CHM, CMT4E, CMT1, CMT1B, CMT2I, CMT2J, CMTDI3, HMSNIB, MPP, Myelin Protein Zero, Charcot-Marie-Tooth Neuropathy 1B, Myelin peripheral protein