PDIA3 ELISA Kit
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- Target Alle PDIA3 ELISA Kits anzeigen
- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A3 (PDIA3). No significant cross-reactivity or interference between Protein Disulfide Isomerase A3 (PDIA3) and analogues was observed.
- Sensitivität
- 0.056 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A3 (PDIA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A3 (PDIA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A3 (PDIA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A3 (PDIA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle PDIA3 ELISA Kits anzeigen
- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
- Andere Bezeichnung
- Protein Disulfide Isomerase A3 (PDIA3 Produkte)
- Synonyme
- ER60 ELISA Kit, ERp57 ELISA Kit, ERp60 ELISA Kit, ERp61 ELISA Kit, GRP57 ELISA Kit, GRP58 ELISA Kit, HsT17083 ELISA Kit, P58 ELISA Kit, PI-PLC ELISA Kit, grp-58 ELISA Kit, 58kDa ELISA Kit, Erp ELISA Kit, Grp58 ELISA Kit, PDI ELISA Kit, PDI-Q2 ELISA Kit, PLC[a] ELISA Kit, Plca ELISA Kit, 1 ELISA Kit, 25D3-membrane-associated ELISA Kit, sb:cb825 ELISA Kit, erp57 ELISA Kit, grp58 ELISA Kit, pdia3 ELISA Kit, protein disulfide isomerase family A member 3 ELISA Kit, protein disulfide-isomerase A3 ELISA Kit, protein disulfide isomerase associated 3 ELISA Kit, protein disulfide isomerase family A, member 3 ELISA Kit, protein disulfide isomerase family A member 3 S homeolog ELISA Kit, PDIA3 ELISA Kit, Tsp_10062 ELISA Kit, Pdia3 ELISA Kit, pdia3 ELISA Kit, pdia3.S ELISA Kit
- Hintergrund
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Gene Name: Protein Disulfide Isomerase A3
Gene Aliases: P58, ERp61, ERp57, ERp60, GRP57, PI-PLC, GRP58, Glucose Regulated Protein,58 kDa, Protein Disulfide Isomerase-Associated 3, Endoplasmic reticulum resident protein 57
- Gen-ID
- 2923
- UniProt
- P30101
- Pathways
- Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
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