F2R ELISA Kit (Coagulation Factor II (thrombin) Receptor)

Details for Product F2R ELISA Kit No. ABIN5658164, Anbieter: Anmelden zum Anzeigen
Antigen
  • CF2R
  • HTR
  • PAR-1
  • PAR1
  • TR
  • Par1
  • TRGPC
  • AI482343
  • Cf2r
  • ThrR
  • Par-1
  • f2r-a
  • par1
  • coagulation factor II thrombin receptor
  • coagulation factor II (thrombin) receptor
  • coagulation factor 2 (thrombin) receptor L homeolog
  • F2R
  • F2r
  • f2r.L
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
19
14
12
5
5
5
5
4
2
2
2
1
Methodentyp
Sandwich ELISA
Detektionsbereich
0.156 ng/mL - 10 ng/mL
Untere Nachweisgrenze
0.156 ng/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Proben Cell Lysate, Tissue Homogenate
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This assay has high sensitivity and excellent specificity for detection of Protease Activated Receptor 1 (PAR1). No significant cross-reactivity or interference between Protease Activated Receptor 1 (PAR1) and analogues was observed.
Sensitivität 0.064 ng/mL
Plasmids, Primers & others Plasmide, Primers & weitere F2R products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Protease Activated Receptor 1 (F2R ELISA Kit Abstract)
Hintergrund

Gene Name: Protease Activated Receptor 1

Gene Aliases: FIIR, F2r, CF2R, HTR, PAR1, TR, Coagulation Factor II Receptor, Thrombin Receptor

Pathways Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
Kommentare

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer 3 h
Plattentyp Pre-coated
Protokoll The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 1 (PAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 1 (PAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 1 (PAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease Activated Receptor 1 (PAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protease Activated Receptor 1 (PAR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protease Activated Receptor 1 (PAR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Haltbarkeit 4-8 months
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