Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Prosaposin ELISA Kit

PSAP Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Seminal Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5658134
  • Target Alle Prosaposin (PSAP) ELISA Kits anzeigen
    Prosaposin (PSAP)
    Reaktivität
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Seminal Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Prosaposin (PSAP). No significant cross-reactivity or interference between Prosaposin (PSAP) and analogues was observed.
    Sensitivität
    0.067 ng/mL
    Featured
    Zu unserem meistverkauften PSAP ELISA Kit
    Top Product
    Discover our top product PSAP ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prosaposin (PSAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prosaposin (PSAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prosaposin (PSAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prosaposin (PSAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prosaposin (PSAP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prosaposin (PSAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Prosaposin (PSAP) ELISA Kits anzeigen
    Prosaposin (PSAP)
    Andere Bezeichnung
    Prosaposin (PSAP Produkte)
    Synonyme
    GLBA ELISA Kit, SAP1 ELISA Kit, AI037048 ELISA Kit, SGP-1 ELISA Kit, SGP1A ELISA Kit, cb759 ELISA Kit, wu:fa14a06 ELISA Kit, wu:fb36e02 ELISA Kit, wu:fb58g07 ELISA Kit, PSAP ELISA Kit, PSP-A ELISA Kit, SFTPA1B ELISA Kit, SP-A ELISA Kit, prosaposin ELISA Kit, surfactant protein A1 ELISA Kit, PSAP ELISA Kit, Psap ELISA Kit, psap ELISA Kit, LOC100011417 ELISA Kit, LOC100304726 ELISA Kit, LOC100526719 ELISA Kit, SFTPA1 ELISA Kit
    Hintergrund

    Gene Name: Prosaposin

    Gene Aliases: SAP1, GLBA, CSAct, Dispersin, Pro-Saposin, Proactivator polypeptide, Glucosylceramidase activator, Sulfatide/GM1 activator, Cerebroside sulfate activator, Sphingolipid Activator 1

    Gen-ID
    5660
    UniProt
    P07602
    Pathways
    Positive Regulation of Endopeptidase Activity
Sie sind hier:
Kundenservice