PML ELISA Kit

Produktnummer ABIN5658122

Produktdetails PML ELISA Kit

Target: PML (Promyelocytic Leukemia (PML))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Promyelocytic Leukemia Protein (PML). No significant cross-reactivity or interference between Promyelocytic Leukemia Protein (PML) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Promyelocytic Leukemia Protein (PML). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Promyelocytic Leukemia Protein (PML). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Promyelocytic Leukemia Protein (PML), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Promyelocytic Leukemia Protein (PML) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Promyelocytic Leukemia Protein (PML) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Promyelocytic Leukemia Protein (PML) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PML

Target: PML (Promyelocytic Leukemia (PML))

Andere Bezeichnung: Promyelocytic Leukemia Protein (PML Produkte)

Synonyme: MYL ELISA Kit, PP8675 ELISA Kit, RNF71 ELISA Kit, TRIM19 ELISA Kit, 1200009E24Rik ELISA Kit, AI661194 ELISA Kit, Trim19 ELISA Kit, RGD1562602 ELISA Kit, PML ELISA Kit, promyelocytic leukemia ELISA Kit, PML ELISA Kit, Pml ELISA Kit

Hintergrund:

Gene Name: Promyelocytic Leukemia Protein

Gene Aliases: MYL, RNF71, TRIM19, Probable Transcription Factor PML, Ring Finger Protein 71, Tripartite motif-containing protein 19

UniProt: P29590

Pathways: p53 Signalweg, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus