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PAOX ELISA Kit

PAOX Reaktivität: Maus Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5657977
  • Target Alle PAOX ELISA Kits anzeigen
    PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))
    Reaktivität
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.12 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.12 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Polyamine Oxidase (PAOX). No significant cross-reactivity or interference between Polyamine Oxidase (PAOX) and analogues was observed.
    Sensitivität
    32 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polyamine Oxidase (PAOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polyamine Oxidase (PAOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polyamine Oxidase (PAOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polyamine Oxidase (PAOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polyamine Oxidase (PAOX) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polyamine Oxidase (PAOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle PAOX ELISA Kits anzeigen
    PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))
    Andere Bezeichnung
    Polyamine Oxidase (PAOX Produkte)
    Synonyme
    PAO ELISA Kit, mpao1 ELISA Kit, 2410012F02Rik ELISA Kit, AI118225 ELISA Kit, Pao ELISA Kit, pao ELISA Kit, polyamine oxidase ELISA Kit, polyamine oxidase 1 ELISA Kit, amine oxidase family protein ELISA Kit, si:dkey-275b16.2 ELISA Kit, polyamine oxidase (exo-N4-amino) ELISA Kit, polyamine oxidase (exo-N4-amino) L homeolog ELISA Kit, PAOX ELISA Kit, pao1 ELISA Kit, NFIA_077590 ELISA Kit, POPTR_0011s12590g ELISA Kit, si:dkey-275b16.2 ELISA Kit, Paox ELISA Kit, paox.L ELISA Kit
    Hintergrund

    Gene Name: Polyamine Oxidase

    Gene Aliases: PAO, Peroxisomal N(1)-Acetyl-Spermine/Spermidine Oxidase(Exo-N4-Amino)

    Gen-ID
    212503
    UniProt
    Q8C0L6
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