PODXL ELISA Kit

Produktnummer ABIN5657958

Produktdetails PODXL ELISA Kit

Target: PODXL (Podocalyxin-Like (PODXL))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Podocalyxin (PCX). No significant cross-reactivity or interference between Podocalyxin (PCX) and analogues was observed.

Sensitivität: 0.59 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocalyxin (PCX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podocalyxin (PCX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podocalyxin (PCX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Podocalyxin (PCX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Podocalyxin (PCX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Podocalyxin (PCX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: Podocalyxin (PODXL Produkte)

Synonyme: Gp200 ELISA Kit, PC ELISA Kit, PCLP ELISA Kit, PCLP-1 ELISA Kit, PCB ELISA Kit, AW121214 ELISA Kit, Ly102 ELISA Kit, Pclp1 ELISA Kit, podocalyxin ELISA Kit, MEP21 ELISA Kit, PODXL ELISA Kit, Gp135 ELISA Kit, PCLP1 ELISA Kit, cPCLP1 ELISA Kit, podxl ELISA Kit, fc18d02 ELISA Kit, wu:fc18d02 ELISA Kit, Pc ELISA Kit, Pcb ELISA Kit, Podxl ELISA Kit, podocalyxin like ELISA Kit, pyruvate carboxylase ELISA Kit, podocalyxin-like ELISA Kit, PODXL ELISA Kit, PC ELISA Kit, Podxl ELISA Kit, podxl ELISA Kit, Pcx ELISA Kit

Hintergrund:

Gene Name: Podocalyxin

Gene Aliases: Gp200, PCLP, PC, PODXL, Podocalyxin Like Protein 1, GCTM-2 antigen

Gen-ID: 192181

UniProt: Q9WTQ2

Pathways: Tube Formation