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PLXNB1 ELISA Kit

PLXNB1 Reaktivität: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
Produktnummer ABIN5657954
  • Target Alle PLXNB1 ELISA Kits anzeigen
    PLXNB1 (Plexin B1 (PLXNB1))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Plexin B1 (PLXNB1). No significant cross-reactivity or interference between Plexin B1 (PLXNB1) and analogues was observed.
    Sensitivität
    0.59 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plexin B1 (PLXNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plexin B1 (PLXNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plexin B1 (PLXNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plexin B1 (PLXNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plexin B1 (PLXNB1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plexin B1 (PLXNB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle PLXNB1 ELISA Kits anzeigen
    PLXNB1 (Plexin B1 (PLXNB1))
    Andere Bezeichnung
    Plexin B1 (PLXNB1 Produkte)
    Synonyme
    im:7054247 ELISA Kit, wu:fb83a09 ELISA Kit, si:ch211-220i18.5 ELISA Kit, 2900002G15Rik ELISA Kit, AU042020 ELISA Kit, mKIAA0407 ELISA Kit, PLEXIN-B1 ELISA Kit, PLXN5 ELISA Kit, SEP ELISA Kit, SET domain containing 5 ELISA Kit, plexin B1 ELISA Kit, plexin b1a ELISA Kit, SETD5 ELISA Kit, PLXNB1 ELISA Kit, plxnb1a ELISA Kit, Plxnb1 ELISA Kit
    Hintergrund

    Gene Name: Plexin B1

    Gene Aliases: PLXN5, SEP, Semaphorin receptor SEP

    Gen-ID
    5364
    UniProt
    O43157
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