PDGFRL ELISA Kit

Produktnummer ABIN5657911

Produktdetails PDGFRL ELISA Kit

Target: PDGFRL (Platelet-Derived Growth Factor Receptor-Like (PDGFRL))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). No significant cross-reactivity or interference between Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) and analogues was observed.

Sensitivität: 30.8 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Receptor Like Protein (PDGFRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PDGFRL

Target: PDGFRL (Platelet-Derived Growth Factor Receptor-Like (PDGFRL))

Andere Bezeichnung: Platelet Derived Growth Factor Receptor Like Protein (PDGFRL Produkte)

Synonyme: zgc:56104 ELISA Kit, zgc:77677 ELISA Kit, MGC82397 ELISA Kit, PDGFRL ELISA Kit, pdgrl ELISA Kit, prlts ELISA Kit, MGC107901 ELISA Kit, PDGRL ELISA Kit, PRLTS ELISA Kit, 1110039P19Rik ELISA Kit, AV013190 ELISA Kit, platelet-derived growth factor receptor-like ELISA Kit, platelet derived growth factor receptor like L homeolog ELISA Kit, platelet derived growth factor receptor like ELISA Kit, pdgfrl ELISA Kit, pdgfrl.L ELISA Kit, PDGFRL ELISA Kit, Pdgfrl ELISA Kit

Hintergrund:

Gene Name: Platelet Derived Growth Factor Receptor Like Protein

Gene Aliases: PRLTS, PDGF-Receptor Beta-Like Tumor Suppressor

Gen-ID: 68797

UniProt: Q6PE55

Pathways: Platelet-derived growth Factor Receptor Signaling