PLSCR1 ELISA Kit

Produktnummer ABIN5657814

Produktdetails PLSCR1 ELISA Kit

Target: PLSCR1 (phospholipid Scramblase 1 (PLSCR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Phospholipid Scramblase 1 (PLSCR1). No significant cross-reactivity or interference between Phospholipid Scramblase 1 (PLSCR1) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipid Scramblase 1 (PLSCR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipid Scramblase 1 (PLSCR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipid Scramblase 1 (PLSCR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipid Scramblase 1 (PLSCR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipid Scramblase 1 (PLSCR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipid Scramblase 1 (PLSCR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PLSCR1

Target: PLSCR1 (phospholipid Scramblase 1 (PLSCR1))

Andere Bezeichnung: Phospholipid Scramblase 1 (PLSCR1 Produkte)

Synonyme: mmtra1b ELISA Kit, MGC84969 ELISA Kit, PLSCR1 ELISA Kit, MGC148322 ELISA Kit, MMTRA1B ELISA Kit, MmTRA1a ELISA Kit, MmTRA1b ELISA Kit, Nor1 ELISA Kit, Tra1 ELISA Kit, Tra1a ELISA Kit, Tra1b ELISA Kit, Tras1 ELISA Kit, Tras2 ELISA Kit, PLSCR2 ELISA Kit, phospholipid scramblase 1 L homeolog ELISA Kit, phospholipid scramblase 1 ELISA Kit, plscr1.L ELISA Kit, PLS1 ELISA Kit, PVX_111580 ELISA Kit, PLSCR1 ELISA Kit, plscr1 ELISA Kit, Plscr1 ELISA Kit, LOC100712949 ELISA Kit, PLSCR2 ELISA Kit, LOC100341366 ELISA Kit, LOC611500 ELISA Kit

Hintergrund:

Gene Name: Phospholipid Scramblase 1

Gene Aliases: MMTRA1B, Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase

Gen-ID: 5359

UniProt: O15162

Pathways: Cellular Response to Molecule of Bacterial Origin