PDE4B ELISA Kit

Produktnummer ABIN5657767

Produktdetails PDE4B ELISA Kit

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 4B, cAMP Specific (PDE4B). No significant cross-reactivity or interference between Phosphodiesterase 4B, cAMP Specific (PDE4B) and analogues was observed.

Sensitivität: 0.065 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 4B, cAMP Specific (PDE4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 4B, cAMP Specific (PDE4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 4B, cAMP Specific (PDE4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 4B, cAMP Specific (PDE4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 4B, cAMP Specific (PDE4B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 4B, cAMP Specific (PDE4B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Andere Bezeichnung: Phosphodiesterase 4B, cAMP Specific (PDE4B Produkte)

Synonyme: dpde4 ELISA Kit, pde4b5 ELISA Kit, pdeivb ELISA Kit, Dpde4 ELISA Kit, R74983 ELISA Kit, dunce ELISA Kit, PDE4/IVb ELISA Kit, Pde4B1 ELISA Kit, Pde4B2 ELISA Kit, Pde4B3 ELISA Kit, Pde4B4 ELISA Kit, DPDE4 ELISA Kit, PDE4B5 ELISA Kit, PDEIVB ELISA Kit, phosphodiesterase 4B ELISA Kit, phosphodiesterase 4B, cAMP specific ELISA Kit, phosphodiesterase 4B S homeolog ELISA Kit, pde4b ELISA Kit, PDE4B ELISA Kit, Pde4b ELISA Kit, pde4b.S ELISA Kit

Hintergrund:

Gene Name: Phosphodiesterase 4B, cAMP Specific

Gene Aliases: DPDE4, PDE32, Dunce(Drosophila)Homolog Phosphodiesterase E4, cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Gen-ID: 24626

UniProt: P14646

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction