PPARA ELISA Kit

Produktnummer ABIN5657732

Produktdetails PPARA ELISA Kit

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Alpha (PPARa). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Alpha (PPARa) and analogues was observed.

Sensitivität: 0.112 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Alpha (PPARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Alpha (PPARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Alpha (PPARa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Alpha (PPARa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Andere Bezeichnung: Peroxisome Proliferator Activated Receptor Alpha (PPARA Produkte)

Synonyme: NR1C1 ELISA Kit, PPAR ELISA Kit, PPARalpha ELISA Kit, hPPAR ELISA Kit, LOC100136415 ELISA Kit, PPARALPHA ELISA Kit, nr1c1 ELISA Kit, xPPARalpha ELISA Kit, xppara ELISA Kit, 4933429D07Rik ELISA Kit, AW742785 ELISA Kit, Nr1c1 ELISA Kit, PPAR-alpha ELISA Kit, Ppar ELISA Kit, peroxisome proliferator activated receptor alpha ELISA Kit, peroxisome proliferator-activated receptor alpha ELISA Kit, peroxisome proliferator activated receptor alpha L homeolog ELISA Kit, PPARA ELISA Kit, LOC100136415 ELISA Kit, ppara.L ELISA Kit, Ppara ELISA Kit, ppara ELISA Kit

Hintergrund:

Gene Name: Peroxisome Proliferator Activated Receptor Alpha

Gene Aliases: NR1C1, PPAR-A, HPPAR, Nuclear Receptor Subfamily 1 Group C Member 1

Gen-ID: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C