PEX2 ELISA Kit

Produktnummer ABIN5657731

Produktdetails PEX2 ELISA Kit

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Peroxisomal Biogenesis Factor 2 (PEX2). No significant cross-reactivity or interference between Peroxisomal Biogenesis Factor 2 (PEX2) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisomal Biogenesis Factor 2 (PEX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisomal Biogenesis Factor 2 (PEX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PEX2

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Andere Bezeichnung: Peroxisomal Biogenesis Factor 2 (PEX2 Produkte)

Synonyme: PAF1 ELISA Kit, PBD5A ELISA Kit, PBD5B ELISA Kit, PMP3 ELISA Kit, PMP35 ELISA Kit, PXMP3 ELISA Kit, RNF72 ELISA Kit, ZWS3 ELISA Kit, D3Ertd138e ELISA Kit, PAF-1 ELISA Kit, Pxmp3 ELISA Kit, pxmp3 ELISA Kit, An18g04150 ELISA Kit, DDBDRAFT_0168812 ELISA Kit, DDBDRAFT_0238045 ELISA Kit, DDB_0168812 ELISA Kit, DDB_0238045 ELISA Kit, peroxisomal biogenesis factor 2 ELISA Kit, RING zinc finger-containing protein ELISA Kit, PEX2 ELISA Kit, Pex2 ELISA Kit, pex2 ELISA Kit, ANI_1_1336164 ELISA Kit, HCAG_04580 ELISA Kit, CC1G_00418 ELISA Kit, PTRG_10616 ELISA Kit, PAAG_05772 ELISA Kit, MGYG_06097 ELISA Kit

Hintergrund:

Gene Name: Peroxisomal Biogenesis Factor 2

Gene Aliases: PXMP3, PAF1, PMP35, PMP3, RNF72, Peroxin 2, Peroxisome Assembly Factor 1, Peroxisomal Membrane Protein 35 kDa, Peroxisomal Membrane Protein 3

Gen-ID: 5828

UniProt: P28328

Pathways: Monocarboxylic Acid Catabolic Process