NPM1 ELISA Kit

Produktnummer ABIN5657473

Produktdetails NPM1 ELISA Kit

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Nucleophosmin (NPM). No significant cross-reactivity or interference between Nucleophosmin (NPM) and analogues was observed.

Sensitivität: 0.119 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleophosmin (NPM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleophosmin (NPM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleophosmin (NPM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleophosmin (NPM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleophosmin (NPM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleophosmin (NPM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: Nucleophosmin (NPM1 Produkte)

Synonyme: B23 ELISA Kit, NPM ELISA Kit, NO38 ELISA Kit, Npm ELISA Kit, B23NP ELISA Kit, npm4 ELISA Kit, npm ELISA Kit, CG7917 ELISA Kit, CRP1 ELISA Kit, Crp1 ELISA Kit, Dmel\\CG7917 ELISA Kit, dNLP ELISA Kit, dNLP-S ELISA Kit, p22 ELISA Kit, npm1 ELISA Kit, sb:cb487 ELISA Kit, nucleophosmin 1 ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog ELISA Kit, nucleophosmin 1b ELISA Kit, Nucleophosmin ELISA Kit, nucleophosmin ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) ELISA Kit, Nucleoplasmin ELISA Kit, nucleophosmin 1a ELISA Kit, NPM1 ELISA Kit, Npm1 ELISA Kit, npm1.L ELISA Kit, npm1b ELISA Kit, npm ELISA Kit, Nlp ELISA Kit, npm1a ELISA Kit

Hintergrund:

Gene Name: Nucleophosmin

Gene Aliases: B23, NPM1, Nucleolar Phosphoprotein B23, Numatrin, Nucleophosmin/Nucleoplasmin Family,Member 1, Nucleolar protein NO38

Gen-ID: 4869

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly