NCOA3 ELISA Kit

Produktnummer ABIN5657459

Produktdetails NCOA3 ELISA Kit

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Nuclear Receptor Coactivator 3 (NCOA3). No significant cross-reactivity or interference between Nuclear Receptor Coactivator 3 (NCOA3) and analogues was observed.

Sensitivität: 0.124 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Coactivator 3 (NCOA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Coactivator 3 (NCOA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Coactivator 3 (NCOA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Coactivator 3 (NCOA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Andere Bezeichnung: Nuclear Receptor Coactivator 3 (NCOA3 Produkte)

Synonyme: NCOA3 ELISA Kit, ACTR ELISA Kit, AIB-1 ELISA Kit, AIB1 ELISA Kit, CAGH16 ELISA Kit, CTG26 ELISA Kit, KAT13B ELISA Kit, RAC3 ELISA Kit, SRC-3 ELISA Kit, SRC3 ELISA Kit, TNRC14 ELISA Kit, TNRC16 ELISA Kit, TRAM-1 ELISA Kit, bHLHe42 ELISA Kit, pCIP ELISA Kit, 2010305B15Rik ELISA Kit, AW321064 ELISA Kit, Actr ELISA Kit, Aib1 ELISA Kit, Rac3 ELISA Kit, Src3 ELISA Kit, Tram-1 ELISA Kit, Tram1 ELISA Kit, p/Cip ELISA Kit, pCip ELISA Kit, actr ELISA Kit, aib-1 ELISA Kit, aib1 ELISA Kit, cagh16 ELISA Kit, ctg26 ELISA Kit, kat13b ELISA Kit, pcip ELISA Kit, rac3 ELISA Kit, src-3 ELISA Kit, src3 ELISA Kit, tnrc14 ELISA Kit, tnrc16 ELISA Kit, tram-1 ELISA Kit, xSRC-3 ELISA Kit, nuclear receptor coactivator 3 ELISA Kit, nuclear receptor coactivator 3 S homeolog ELISA Kit, NCOA3 ELISA Kit, Ncoa3 ELISA Kit, ncoa3.S ELISA Kit

Hintergrund:

Gene Name: Nuclear Receptor Coactivator 3

Gene Aliases: RAC3, ACTR, AIB1, CAGH16, CTG26, SRC3, TNRC14, TNRC16, TRAM1, pCIP, Amplified In Breast 1, Steroid Receptor Coactivator-3 , Thyroid Hormone Receptor Activator Molecule 1

Gen-ID: 8202

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha