NKAP ELISA Kit

Produktnummer ABIN5657374

Produktdetails NKAP ELISA Kit

Target: NKAP (NFKB Activating Protein (NKAP))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of NFKB Activating Protein (NKAP). No significant cross-reactivity or interference between NFKB Activating Protein (NKAP) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB Activating Protein (NKAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFKB Activating Protein (NKAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB Activating Protein (NKAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NFKB Activating Protein (NKAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NFKB Activating Protein (NKAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NFKB Activating Protein (NKAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NKAP

Target: NKAP (NFKB Activating Protein (NKAP))

Andere Bezeichnung: NFKB Activating Protein (NKAP Produkte)

Synonyme: DSMA4 ELISA Kit, GEF720 ELISA Kit, Syx ELISA Kit, Tech ELISA Kit, c6orf194l ELISA Kit, wu:fj36d09 ELISA Kit, wu:fj49c09 ELISA Kit, 2610020O08Rik ELISA Kit, AA987160 ELISA Kit, AI849147 ELISA Kit, AL024236 ELISA Kit, 2610020o08rik ELISA Kit, nkap ELISA Kit, NKAPL ELISA Kit, NFKB activating protein ELISA Kit, pleckstrin homology and RhoGEF domain containing G5 ELISA Kit, NFKB activating protein S homeolog ELISA Kit, NKAP ELISA Kit, PLEKHG5 ELISA Kit, nkap ELISA Kit, Nkap ELISA Kit, nkap.S ELISA Kit

Hintergrund:

Gene Name: NFKB Activating Protein

Gene Aliases: NF Kappa B Activating Protein

Pathways: Stem Cell Maintenance, Phosphorylierungen bei SARS-CoV-2 Infektion