Neuropilin 1 ELISA Kit (NRP1)

Details for Product NRP1 ELISA Kit No. ABIN5657327, Anbieter: Anmelden zum Anzeigen
Antigen
  • NRP1
  • cd304
  • neuropilin
  • np-1
  • npn-1
  • nrp
  • nrp-1
  • vegf165r
  • BDCA4
  • CD304
  • NP1
  • NRP
  • VEGF165R
  • C530029I03
  • NP-1
  • NPN-1
  • Npn1
  • Nrp
  • neuropilin 1
  • neuropilin 1 L homeolog
  • NRP1
  • nrp1
  • Nrp1
  • nrp1.L
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
20
16
14
3
2
2
2
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5 pg/mL - 4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Proben Plasma, Serum, Tissue Homogenate
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This assay has high sensitivity and excellent specificity for detection of Neuropilin 1 (NRP1). No significant cross-reactivity or interference between Neuropilin 1 (NRP1) and analogues was observed.
Sensitivität 26.2 pg/mL
Plasmids, Primers & others Plasmide, Primers & weitere Neuropilin 1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Neuropilin 1 (NRP1 ELISA Kit Abstract)
Hintergrund

Gene Name: Neuropilin 1

Gene Aliases: CD304, BDCA4, VEGF165R, Vascular endothelial cell growth factor 165 receptor
Gen-ID 8829
UniProt O14786
Pathways Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
Kommentare

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Testdauer 3 h
Plattentyp Pre-coated
Protokoll The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropilin 1 (NRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropilin 1 (NRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropilin 1 (NRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropilin 1 (NRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Haltbarkeit 4-8 months
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