NEFL ELISA Kit

Produktnummer ABIN5657277

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus NEFL.

Kurzübersicht für NEFL ELISA Kit (ABIN5657277)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 4.94 pg/mL - 400 pg/mL

Applikation: ELISA

Proben: Cerebrospinal Fluid, Plasma, Serum

Produktdetails NEFL ELISA Kit

Untere Nachweisgrenze: 4.94 pg/mL

Verwendungszweck: Mouse Neurofilament, Light Polypeptide (NEFL) High-sensitive ELISA Kit for detecting NEFL in serum, plasma, cerebrospinal fluid

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

Sensitivität: 1.87 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Andere Bezeichnung: Neurofilament, Light Polypeptide

Hintergrund: Gene Aliases: CMT1F, CMT2E, NF-L, NF68, NFL, 68 kDa neurofilament protein, Neurofilament triplet L protein

Gen-ID: 18039

UniProt: P08551