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NELL2 ELISA Kit

NELL2 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5657202
  • Target Alle NELL2 ELISA Kits anzeigen
    NELL2 (NEL-Like 2 (Chicken) (NELL2))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of NEL Like Protein 2 (NELL2). No significant cross-reactivity or interference between NEL Like Protein 2 (NELL2) and analogues was observed.
    Sensitivität
    0.116 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEL Like Protein 2 (NELL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEL Like Protein 2 (NELL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEL Like Protein 2 (NELL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEL Like Protein 2 (NELL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEL Like Protein 2 (NELL2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEL Like Protein 2 (NELL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle NELL2 ELISA Kits anzeigen
    NELL2 (NEL-Like 2 (Chicken) (NELL2))
    Andere Bezeichnung
    NEL Like Protein 2 (NELL2 Produkte)
    Synonyme
    A330108N19Rik ELISA Kit, R75516 ELISA Kit, mel91 ELISA Kit, nel ELISA Kit, NRP2 ELISA Kit, nell2 ELISA Kit, wu:fj43h11 ELISA Kit, zgc:158375 ELISA Kit, neural EGFL like 2 L homeolog ELISA Kit, NEL-like 2 ELISA Kit, neural EGFL like 2 ELISA Kit, neural EGFL like 2a ELISA Kit, nell2.L ELISA Kit, Nell2 ELISA Kit, NELL2 ELISA Kit, nell2a ELISA Kit
    Hintergrund

    Gene Name: NEL Like Protein 2

    Gene Aliases: NRP2, Nel-related protein 2, Protein kinase C-binding protein NELL2

    Gen-ID
    4753
    UniProt
    Q99435
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