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MFN2 ELISA Kit

MFN2 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5656921
  • Target Alle MFN2 ELISA Kits anzeigen
    MFN2 (Mitofusin 2 (MFN2))
    Reaktivität
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Mitofusin 2 (MFN2). No significant cross-reactivity or interference between Mitofusin 2 (MFN2) and analogues was observed.
    Sensitivität
    0.055 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitofusin 2 (MFN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitofusin 2 (MFN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitofusin 2 (MFN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitofusin 2 (MFN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitofusin 2 (MFN2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitofusin 2 (MFN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle MFN2 ELISA Kits anzeigen
    MFN2 (Mitofusin 2 (MFN2))
    Andere Bezeichnung
    Mitofusin 2 (MFN2 Produkte)
    Synonyme
    CG3869 ELISA Kit, Dmel\\CG3869 ELISA Kit, MARF ELISA Kit, Marf-1 ELISA Kit, Mfn ELISA Kit, anon-WO0125274.3 ELISA Kit, dMFN ELISA Kit, dMfn ELISA Kit, dmfn ELISA Kit, marf ELISA Kit, mfn ELISA Kit, mfn2 ELISA Kit, MFN2 ELISA Kit, hsg ELISA Kit, cmt2a ELISA Kit, cprp1 ELISA Kit, cmt2a2 ELISA Kit, CMT2A ELISA Kit, CMT2A2 ELISA Kit, CPRP1 ELISA Kit, HSG ELISA Kit, D630023P19Rik ELISA Kit, Fzo ELISA Kit, mg:cb01g09 ELISA Kit, si:dkeyp-104h9.2 ELISA Kit, wu:fb79a11 ELISA Kit, mitofusin 2 ELISA Kit, Mitochondrial assembly regulatory factor ELISA Kit, mitofusin-2 ELISA Kit, mitofusin 2 L homeolog ELISA Kit, MFN2 ELISA Kit, Marf ELISA Kit, mfn2 ELISA Kit, LOC100186475 ELISA Kit, Mfn2 ELISA Kit, mfn2.L ELISA Kit
    Hintergrund

    Gene Name: Mitofusin 2

    Gene Aliases: CMT2A, CMT2A2, CPRP1, HSG, MARF, Transmembrane GTPase MFN2

    Gen-ID
    9927
    UniProt
    O95140
    Pathways
    Skeletal Muscle Fiber Development
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