MAT2A ELISA Kit

Produktnummer ABIN5656862

Produktdetails MAT2A ELISA Kit

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Methionine Adenosyltransferase II Alpha (MAT2a). No significant cross-reactivity or interference between Methionine Adenosyltransferase II Alpha (MAT2a) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MAT2A

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Andere Bezeichnung: Methionine Adenosyltransferase II Alpha (MAT2A Produkte)

Synonyme: MATA2 ELISA Kit, MATII ELISA Kit, SAMS2 ELISA Kit, Sams2 ELISA Kit, D630045P18Rik ELISA Kit, fd12a12 ELISA Kit, mat2a ELISA Kit, si:ch73-340n8.1 ELISA Kit, wu:fb95e01 ELISA Kit, wu:fd12a12 ELISA Kit, m(2)21ab ELISA Kit, MGC76253 ELISA Kit, MGC79598 ELISA Kit, zgc:110847 ELISA Kit, methionine adenosyltransferase 2A ELISA Kit, methionine adenosyltransferase II, alpha ELISA Kit, methionine adenosyltransferase II, alpha a ELISA Kit, methionine adenosyltransferase II, alpha L homeolog ELISA Kit, methionine adenosyltransferase II, alpha b ELISA Kit, MAT2A ELISA Kit, Mat2a ELISA Kit, mat2aa ELISA Kit, mat2a.L ELISA Kit, mat2a ELISA Kit, mat2ab ELISA Kit

Hintergrund:

Gene Name: Methionine Adenosyltransferase II Alpha

Gene Aliases: SAMS2, MATA2, AMS2, MATII, S-adenosylmethionine synthase isoform type-2

Gen-ID: 4144

UniProt: P31153

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process