MIF CLIA Kit
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- Target Alle MIF CLIA Kits anzeigen
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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Reaktivität
- Maus
- Nachweismethode
- Chemiluminescent
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 156.25 pg/mL - 10000 pg/mL
- Untere Nachweisgrenze
- 156.25 pg/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Macrophage Migration Inhibitory Factor (MIF). No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.
- Sensitivität
- 6.4 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 - 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor (MIF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor (MIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Migration Inhibitory Factor (MIF) level in the sample or standard.,
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle MIF CLIA Kits anzeigen
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
- Andere Bezeichnung
- Macrophage Migration Inhibitory Factor (MIF Produkte)
- Synonyme
- mif CLIA Kit, Mif CLIA Kit, gif CLIA Kit, glif CLIA Kit, mmif CLIA Kit, LOC100136498 CLIA Kit, LOC100284350 CLIA Kit, LOC100284546 CLIA Kit, GIF CLIA Kit, Glif CLIA Kit, GLIF CLIA Kit, MMIF CLIA Kit, macrophage migration inhibitory factor L homeolog CLIA Kit, macrophage migration inhibitory factor CLIA Kit, macrophage migration inhibitory factor (glycosylation-inhibiting factor) CLIA Kit, mif.L CLIA Kit, mif CLIA Kit, MIF CLIA Kit, Mif CLIA Kit, PHATRDRAFT_49660 CLIA Kit, LOC100136498 CLIA Kit, cl405_1 CLIA Kit, LOC100284546 CLIA Kit
- Hintergrund
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Gene Name: Macrophage Migration Inhibitory Factor
Gene Aliases: GIF, GLIF, MMIF, Glycosylation-Inhibiting Factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase
- Gen-ID
- 17319
- UniProt
- P34884
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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