CCL20 ELISA Kit
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- Target Alle CCL20 ELISA Kits anzeigen
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 7.81 pg/mL - 500 pg/mL
- Untere Nachweisgrenze
- 7.81 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) and analogues was observed.
- Sensitivität
- 2.7 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Alpha (MIP3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle CCL20 ELISA Kits anzeigen
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
- Andere Bezeichnung
- Macrophage Inflammatory Protein 3 Alpha (CCL20 Produkte)
- Synonyme
- MIP-3ALPHA ELISA Kit, CCL20 ELISA Kit, si:dkey-150o13.1 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP-3-alpha ELISA Kit, MIP-3a ELISA Kit, MIP3A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, MIP-3A ELISA Kit, MIP-3[a] ELISA Kit, Scya20 ELISA Kit, exodus-1 ELISA Kit, C-C motif chemokine ligand 20 ELISA Kit, chemokine (C-C motif) ligand 20b ELISA Kit, chemokine (C-C motif) ligand 20 ELISA Kit, CCL20 ELISA Kit, ccl20b ELISA Kit, Ccl20 ELISA Kit
- Hintergrund
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Gene Name: Macrophage Inflammatory Protein 3 Alpha
Gene Aliases: CCL20, CKb4, LARC, MIP3-A, SCYA20, ST38, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20, Beta-Chemokine Exodus-1, Liver And Activation-Regulated Chemokine
- Gen-ID
- 6364
- UniProt
- P78556
- Pathways
- Phosphorylierungen bei SARS-CoV-2 Infektion
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