Luteinizing Hormone CLIA Kit

Produktnummer ABIN5656511

Ratte Luteinizing Hormone ELISA Kit, Chemiluminescent Assay für die Quantifizierung von Ratte Luteinizing Hormone.

Kurzübersicht für Luteinizing Hormone CLIA Kit (ABIN5656511)

Target: Luteinizing Hormone (LH)

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 195 pg/mL - 50000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Luteinizing Hormone CLIA Kit

Untere Nachweisgrenze: 195 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH). No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

Sensitivität: 70 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Luteinizing Hormone (LH). A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Luteinizing Hormone (LH) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Luteinizing Hormone (LH)

Andere Bezeichnung: Luteinizing Hormone

Substanzklasse: Hormone

Hintergrund:

Gene Name: Luteinizing Hormone

Gene Aliases: ICSH, Lutropin, Interstitial Cell Stimulating Hormone