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LAMB2 ELISA Kit

LAMB2 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5656317
  • Target Alle LAMB2 ELISA Kits anzeigen
    LAMB2 (Laminin, beta 2 (Laminin S) (LAMB2))
    Reaktivität
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.78 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.78 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Laminin Beta 2 (LAMb2). No significant cross-reactivity or interference between Laminin Beta 2 (LAMb2) and analogues was observed.
    Sensitivität
    0.31 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Beta 2 (LAMb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Beta 2 (LAMb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Beta 2 (LAMb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Beta 2 (LAMb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Beta 2 (LAMb2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Beta 2 (LAMb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LAMB2 ELISA Kits anzeigen
    LAMB2 (Laminin, beta 2 (Laminin S) (LAMB2))
    Andere Bezeichnung
    Laminin Beta 2 (LAMB2 Produkte)
    Synonyme
    LAMS ELISA Kit, NPHS5 ELISA Kit, AW211941 ELISA Kit, Lamb-2 ELISA Kit, Lams ELISA Kit, SLAM ELISA Kit, lamb1 ELISA Kit, lams ELISA Kit, LAMC1 ELISA Kit, laminin ELISA Kit, laminin subunit beta 2 ELISA Kit, laminin, beta 2 ELISA Kit, laminin subunit beta 2 L homeolog ELISA Kit, laminin, beta 2 (laminin S) ELISA Kit, LAMB2 ELISA Kit, Lamb2 ELISA Kit, lamb2.L ELISA Kit
    Hintergrund

    Gene Name: Laminin Beta 2

    Gene Aliases: LAM-B2, LAMS, Laminin S, Laminin B1s chain, S-laminin subunit beta

    Gen-ID
    3913
    UniProt
    P55268
    Pathways
    Skeletal Muscle Fiber Development
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