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KPNB1 ELISA Kit

KPNB1 Reaktivität: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5656173
  • Target Alle KPNB1 ELISA Kits anzeigen
    KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.781 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.781 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Karyopherin Beta (KPNb1). No significant cross-reactivity or interference between Karyopherin Beta (KPNb1) and analogues was observed.
    Sensitivität
    0.28 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Beta (KPNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Beta (KPNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Beta (KPNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Beta (KPNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Karyopherin Beta (KPNb1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Karyopherin Beta (KPNb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle KPNB1 ELISA Kits anzeigen
    KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
    Andere Bezeichnung
    Karyopherin Beta (KPNB1 Produkte)
    Synonyme
    Afu1g15720 ELISA Kit, AO090005001133 ELISA Kit, imb1 ELISA Kit, impnb ELISA Kit, ipo1 ELISA Kit, ipob ELISA Kit, ntf97 ELISA Kit, IMB1 ELISA Kit, IPO1 ELISA Kit, IPOB ELISA Kit, Impnb ELISA Kit, NTF97 ELISA Kit, AA409963 ELISA Kit, importin beta-1 subunit ELISA Kit, Importin beta-1 subunit ELISA Kit, karyopherin (importin) beta 1 L homeolog ELISA Kit, importin beta 1 ELISA Kit, karyopherin subunit beta 1 ELISA Kit, karyopherin (importin) beta 1 ELISA Kit, AFUA_1G15720 ELISA Kit, AOR_1_1974174 ELISA Kit, Bm1_57540 ELISA Kit, PMAA_065830 ELISA Kit, AFLA_083750 ELISA Kit, TSTA_085870 ELISA Kit, BDBG_07509 ELISA Kit, MCYG_00039 ELISA Kit, kpnb1.L ELISA Kit, LOC100533387 ELISA Kit, KPNB1 ELISA Kit, Kpnb1 ELISA Kit
    Hintergrund

    Gene Name: Karyopherin Beta

    Gene Aliases: IMB1, IPOB, Impnb, Impnb, IPO1, NTF97, PTAC97, Importin subunit beta-1, Importin-90, Nuclear factor p97, Pore targeting complex 97 kDa subunit

    Gen-ID
    3837
    UniProt
    Q14974
    Pathways
    Protein targeting to Nucleus
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