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ICAM5 ELISA Kit

ICAM5 Reaktivität: Maus Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5655538
  • Target Alle ICAM5 ELISA Kits anzeigen
    ICAM5 (Intercellular Adhesion Molecule 5 (ICAM5))
    Reaktivität
    • 4
    • 2
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 5 (ICAM5). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 5 (ICAM5) and analogues was observed.
    Sensitivität
    0.62 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 5 (ICAM5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 5 (ICAM5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 5 (ICAM5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intercellular Adhesion Molecule 5 (ICAM5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 5 (ICAM5) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 5 (ICAM5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ICAM5 ELISA Kits anzeigen
    ICAM5 (Intercellular Adhesion Molecule 5 (ICAM5))
    Andere Bezeichnung
    Intercellular Adhesion Molecule 5 (ICAM5 Produkte)
    Synonyme
    TLCN ELISA Kit, TLN ELISA Kit, CD50 ELISA Kit, ICAM-3 ELISA Kit, Icam3 ELISA Kit, Tlcn ELISA Kit, ICAM5 ELISA Kit, icam5 ELISA Kit, tlcn ELISA Kit, tln ELISA Kit, intercellular adhesion molecule 5 ELISA Kit, intercellular adhesion molecule 5, telencephalin ELISA Kit, intercellular adhesion molecule 5 L homeolog ELISA Kit, ICAM5 ELISA Kit, Icam5 ELISA Kit, LOC100511183 ELISA Kit, icam5.L ELISA Kit
    Hintergrund

    Gene Name: Intercellular Adhesion Molecule 5

    Gene Aliases: TLCN, TLN, Telencephalin

    Gen-ID
    15898
    UniProt
    Q60625
    Pathways
    Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location
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