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ITGB1 ELISA Kit

ITGB1 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Produktnummer ABIN5655500
  • Target Alle ITGB1 ELISA Kits anzeigen
    ITGB1 (Integrin beta 1 (ITGB1))
    Reaktivität
    • 8
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.78 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.78 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Integrin Beta 1 (ITGb1). No significant cross-reactivity or interference between Integrin Beta 1 (ITGb1) and analogues was observed.
    Sensitivität
    0.28 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 1 (ITGb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 1 (ITGb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 1 (ITGb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 1 (ITGb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 1 (ITGb1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 1 (ITGb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ITGB1 ELISA Kits anzeigen
    ITGB1 (Integrin beta 1 (ITGB1))
    Andere Bezeichnung
    Integrin Beta 1 (ITGB1 Produkte)
    Synonyme
    CD29 ELISA Kit, FNRB ELISA Kit, GPIIA ELISA Kit, MDF2 ELISA Kit, MSK12 ELISA Kit, VLA-BETA ELISA Kit, VLAB ELISA Kit, 4633401G24Rik ELISA Kit, AA409975 ELISA Kit, AA960159 ELISA Kit, ENSMUSG00000051907 ELISA Kit, Fnrb ELISA Kit, Gm9863 ELISA Kit, gpIIa ELISA Kit, cd29 ELISA Kit, fnrb ELISA Kit, gpiia ELISA Kit, itgb1 ELISA Kit, mdf2 ELISA Kit, msk12 ELISA Kit, vla-beta ELISA Kit, vlab ELISA Kit, itgb1-a ELISA Kit, itgb1-b ELISA Kit, integrin subunit beta 1 ELISA Kit, integrin beta 1 (fibronectin receptor beta) ELISA Kit, integrin subunit beta 1 S homeolog ELISA Kit, integrin beta 1 ELISA Kit, integrin subunit beta 1 L homeolog ELISA Kit, ITGB1 ELISA Kit, Itgb1 ELISA Kit, itgb1.S ELISA Kit, LOC100009173 ELISA Kit, itgb1.L ELISA Kit
    Hintergrund

    Gene Name: Integrin Beta 1

    Gene Aliases: CD29, MSK12, ITG-B1, FNRB, GPIIA, MDF2, MSK12, VLAB, Fibronectin Receptor Beta Polypeptide, Antigen CD29 Includes MDF2, Glycoprotein IIa, VLA-4 subunit beta

    Gen-ID
    3688
    UniProt
    P05556
    Pathways
    Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interaktom
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