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Hepcidin ELISA Kit

HAMP Reaktivität: Schwein Colorimetric Competition ELISA 740.74 pg/mL - 60000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5655097
  • Target Alle Hepcidin (HAMP) ELISA Kits anzeigen
    Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
    Reaktivität
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Schwein
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    740.74 pg/mL - 60000 pg/mL
    Untere Nachweisgrenze
    740.74 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc). No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.
    Sensitivität
    283.51 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hepcidin (Hepc) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hepcidin (Hepc) and unlabeled Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to Hepcidin (Hepc). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hepcidin (Hepc) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hepcidin (Hepc) in the sample.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Hepcidin (HAMP) ELISA Kits anzeigen
    Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
    Andere Bezeichnung
    Hepcidin (HAMP Produkte)
    Synonyme
    HEPC ELISA Kit, HFE2B ELISA Kit, LEAP1 ELISA Kit, PLTR ELISA Kit, Hepcidin ELISA Kit, Hamp1 ELISA Kit, Hepc ELISA Kit, Hepc1 ELISA Kit, HAMP ELISA Kit, hepcidin ELISA Kit, hamp1 ELISA Kit, hep1 ELISA Kit, hepcidin antimicrobial peptide ELISA Kit, hepcidin-like ELISA Kit, hepcidin-1 ELISA Kit, Ham percentage ELISA Kit, HAMP ELISA Kit, Hamp ELISA Kit, hamp ELISA Kit, LOC100135935 ELISA Kit, LOC106584587 ELISA Kit
    Hintergrund

    Gene Name: Hepcidin

    Gene Aliases: HAMP, HFE2B, PLTR, LEAP1, Hepcidin Antimicrobial Peptide, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor

    UniProt
    Q8MJ80
    Pathways
    Hormone Activity, Transition Metal Ion Homeostasis
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