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GHRH ELISA Kit

GHRH Reaktivität: Human Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5654906
  • Target Alle GHRH ELISA Kits anzeigen
    GHRH (Growth Hormone Releasing Hormone (GHRH))
    Reaktivität
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    12.35 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    12.35 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH). No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.
    Sensitivität
    4.92 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone Releasing Hormone (GHRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone Releasing Hormone (GHRH) and unlabeled Growth Hormone Releasing Hormone (GHRH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone Releasing Hormone (GHRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle GHRH ELISA Kits anzeigen
    GHRH (Growth Hormone Releasing Hormone (GHRH))
    Andere Bezeichnung
    Growth Hormone Releasing Hormone (GHRH Produkte)
    Synonyme
    GHRF ELISA Kit, GRF ELISA Kit, INN ELISA Kit, PACAP ELISA Kit, grf ELISA Kit, Ghrf ELISA Kit, growth hormone releasing hormone ELISA Kit, adenylate cyclase activating polypeptide 1 ELISA Kit, GHRH ELISA Kit, ADCYAP1 ELISA Kit, ghrh ELISA Kit, Ghrh ELISA Kit, LOC780526 ELISA Kit
    Hintergrund

    Gene Name: Growth Hormone Releasing Hormone

    Gene Aliases: GRF, GHRF, Somatocrinin, Growth-Hormone-Releasing Factor, Sermorelin, Somatoliberin

    Gen-ID
    2691
    UniProt
    P01286
    Pathways
    Hormone Transport, Hormone Activity, cAMP Metabolic Process
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