Glutamic Acid Decarboxylase ELISA Kit

Produktnummer ABIN5654675

Human Glutamic Acid Decarboxylase ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Glutamic Acid Decarboxylase.

Kurzübersicht für Glutamic Acid Decarboxylase ELISA Kit (ABIN5654675)

Target: Glutamic Acid Decarboxylase (GAD)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Glutamic Acid Decarboxylase ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Human Glutamic Acid Decarboxylase (Anti-GAD) Anti-antigen ELISA Kit for detecting Anti-GAD in serum, plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glutamic Acid Decarboxylase (GAD). No significant cross-reactivity or interference between Glutamic Acid Decarboxylase (GAD) and analogues was observed.

Sensitivität: 0.59 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 2.5 h

Plattentyp: Pre-coated

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamic Acid Decarboxylase (GAD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamic Acid Decarboxylase (GAD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Glutamic Acid Decarboxylase

Target: Glutamic Acid Decarboxylase (GAD)

Andere Bezeichnung: Glutamic Acid Decarboxylase

Hintergrund: Gene Aliases: Glutamate Decarboxylase

Gen-ID: 2571

UniProt: Q99259